Meta-analytical review of antioxidant mechanisms responses in animals exposed to herbicide 2,4-D herbicide.

The 2,4-Dichlorophenoxyacetic acid (2,4-D) is a low-cost herbicide to eradicate broadleaf weeds. Since the development of 2,4-D resistant transgenic crops, it has been described as one of the most widely distributed pollutants in the world, increasing concern about its environmental impacts. This study aimed to elucidate the antioxidant system response in animals exposed to 2,4-D by different routes of exposure. It focused on determining if tissue, phylogenetic group, and herbicide formulation would influence the antioxidant mechanisms. A careful literature search of Scopus, WoS, and Science Direct retrieved 6983, 24,098, and 20,616 articles, respectively. The dataset comprised 390 control-treatment comparisons and included three routes of exposure: transgenerational, oral, and topical. The data set for transgenerational and oral exposure revealed oxidative stress through a decrease in enzymatic activities and the level of molecules of the antioxidant system. In contrast, topical exposure increased the oxidative stress. Tissue-specific analyses revealed that the transgenerational effects reduced hepatic catalase (CAT) activity. Oral exposure caused a variety of effects, including increased CAT activity in the prostate and decreased activity in various tissues. Mammals predominate in the transgenerational and oral groups, showing a significantly reduced activity of the antioxidant system. In contrast, in the topical exposure, an increased activity of oxidative stress biomarkers was observed in fish, earthworms, and mollusks. The effects of the 2,4-D formulation on oxidative stress responses showed significant differences between pure and commercial formulations, with oral exposure resulting in decreased activity and topical exposure increasing responses. In summary, orally exposed animals exhibited a clear decrease in enzyme activities, transgenerational exposure elicited tissue-specific prompted biochemical reductions, and topical exposure induced increased responses, emphasizing the need for unbiased exploration of the effects of 2,4-D on biomarkers of oxidative stress while addressing publication bias in oral and topical datasets.

Comparative effects of oral exposure to 2, 4, 6-tribromophenol and decabromodiphenyl ether in Nile tilapia.

Flame retardants are compounds added to a high diversity of polymers used in electronics and furniture to decrease the risk of combustion. Decabromodiphenyl ether (BDE-209) is a polybrominated diphenyl ether (PBDE) congener still used worldwide, while 2, 4, 6-tribromophenol(TBP) is a PBDE metabolite used as a pesticide and flame retardant for wood conservation. These compounds have lipophilic properties and are easily bioaccumulated in the food chain. In the current study, the acute and chronic toxicity of BDE-209 and TBP was evaluated in Oreochromis niloticus through analyses of redox imbalance, neurotoxicity, and histopathological biomarkers after acute (24, 48, 72, and 96 h) and chronic (90 days) exposure to 0.5, 5, and 50 ng/g of the polybrominated compounds. The results showed effects on glutathione S-transferase(GST) activity and damage to biomolecules in both acute and chronic exposures. Liver histopathology and the ultrastructure of hepatocytes revealed alterations and damage in individuals from both experiments, but only BDE-209 led to neurotoxic effects. The current study revealed new endpoints related to polybrominated compounds in fish, highlighting the need to review the risk of exposure to biota.

Effects of trophic 2,2', 4,4'-tetrabromodiphenyl ether (BDE-47) exposure in Oreochromis niloticus: A multiple biomarkers analysis.

Polybrominated diphenyl esters are emerging environmental contaminants with few toxicological data, being a concern for the scientific community. This study evaluated the effects of BDE-47 on the health of Oreochromis niloticus fish. The animals were exposed to three doses of BDE-47 (0, 0.253, 2.53, 25.3 ng g-1) every 10 days, for 80 days. The BDE-47 affected the hepatosomatic and gonadosomatic index in female and the condition factor by intermediate dose in both sexes. The levels of estradiol decreased and the T4 are increased, but the vitellogenin production was not modulated in male individuals. Changes in AChE, GST, LPO and histopathology were observed while the integrated biomarker response index suggests that the lowest dose of BDE-47 compromised the activity of antioxidant enzymes. The oral exposure to BDE-47 in environmental concentrations is toxic to O. niloticus and the use of multiple biomarkers is an attribution in ecotoxicology studies and biomonitoring programs.

Effects of different filtration techniques on quality and toxicology of post treatment effluent from an anaerobic reactor.

The early stages of the fish life cycle correspond to the phase that is most susceptible to deleterious effects caused by exposure to pollution. The aim of this study was to evaluate the quality of the anaerobic reactor effluent after three filtration-based treatments, namely, Double-Filtration in gravel and sand (DF), Triple-Filtration in gravel, sand and Activated Charcoal (TF-AC) and Triple-Filtration in gravel, sand and clinoptilolite (TF-C). The toxic effects on the population dynamics of larvae and embryos of catfish (Rhamdia quelen) to the final effluent were evaluated using an individual-based model (IBM). The results indicate that the three post-treatments produced effluents with significant improvement of the physicochemical parameters evaluated in relation to the anaerobic reactor effluent. In addition, all post-treatments improved the removal of metal ions. Experimental data showed high mortality rates for Rhamdia quelen embryos and larvae for most treatments, except for the effluent treated by TF-C. The results demonstrated that the concentration of ammoniacal nitrogen in relation to AR treatments (69.0); DF (44.0); TF-AC (46.6) and TF-C (0.33) in mg/L can be a limiting factor for the development of embryos and larvae during the ecotoxicity tests.

Waterborn Genotoxicity in Southern Brazil Using Astyanax bifasciatus (Pisces: Teleostei).

Water bodies are often negatively affected by unmediated expansion of surrounding human populations. This makes it essential to establish growth planning strategies that balance productivity and sustainability when creating a viable ecological equilibrium. This study aimed to evaluate genotoxic effects in southern Brazil, using biomarkers in the fish Astyanax bifasciatus, during summer and winter of 2016. The erythrocytic nuclear abnormalities test and the micronucleus test as well as the blood and liver comet assay were used to determine genetic biomarker damage. Four locations and a control point (CP) were sampled in this study. The results demonstrated genotoxicity at all sample locations in the river as compared to the CP in all tests. This is concerning as this water source is the only supply for human populations adjacent and further flows into the greater Iguaçu river basin.

Does exposure to glyphosate lead to an increase in the micronuclei frequency? A systematic and meta-analytic review.

Glyphosate-based herbicides are among the most used pesticides worldwide. Reviews on the safety of glyphosate have been conducted by several regulatory agencies and researches centers, many times with contradictory results. This study is a systematic meta-analytical review of experimental studies on the relationship between exposure to the glyphosate (GLY) and its formulations with the formation of micronuclei (MN) to establish a quantitative estimate of the environmental risks. The natural logarithm (ln) of the estimated response ratio was calculated from 81 experiments. A meta-analysis was performed on the complete data set, and individual meta-analyses were conducted after stratification by test system, class of vertebrate, exposure route, gender, endpoints, type of literature, formulation, GLY dose and exposure time. A forest plot showed an overall positive association between GLY exposure and its formulations and MN, corroborated by the cumulative effects size. Different responses were observed on mammalian and non-mammalian. Interesting results was noticed in exposure route where oral administration of GLY presented no significance. Exposure by intraperitoneal injection presented the highest MN formation. Pure GLY caused fewer effects than to commercial mixtures, but both presented mutagenic effects. The studies with males presented significant responses, while studies with females were not significant. The cumulative effects size was not clearly related to GLY dose, and was negatively related to exposure time. It can be attributed to different test systems, exposure routes and protocols analyzed. In conclusion, our results support the hypothesis that exposure to GLY and its formulations increases the frequency of MN formation.

In situ assessment of a neotropical fish to evaluate pollution in a river receiving agricultural and urban wastewater.

We aimed to assess the quality of a midsize river that receives agricultural and urban wastewater. Nuclear abnormalities (NA), comet assays of blood and gills, and gill histopathology were evaluated in fish Astyanax aff. paranae during the summer and winter 2011 at three sites in Paraná State, Brazil: (1) a biological reserve (Rebio-reference area); (2) an agricultural site; (3) a downstream site that accumulates agricultural and urban effluents. We found the highest effects of pollutants in fish at the downstream site during the summer. The agricultural site showed an intermediate damage rate, and fish at Rebio generally had the least damage, with the exception of NA. Despite conflicting results from the biomarkers used, we observed an increase in damage associated with the accumulation of pollutants. Pesticides are probable xenobiotics in the agricultural area. Additionally, metals and substances such as pharmaceuticals and ammonia may be present at the downstream site.